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Accueil du site > Production scientifique > Structural characterization and biological properties of a novel N-acylated lipooligosaccharide (LOS-IV) in Mycobacterium marinum

Structural characterization and biological properties of a novel N-acylated lipooligosaccharide (LOS-IV) in Mycobacterium marinum

Date de publication: 21 octobre 2010

Y. Rombouts, E. Elass, C. Biot, E. Maes, B. Coddeville, A. Burguiere, C. Tokarski, E. Buisine, X. Trivelli, L. Kremer, Y. Guerardel
J. Am. Chem. Soc. 132 16073 (2010). DOI

Travail réalisé sur le site de l’Université de Lille 1, Sciences et Technologies.

Abstract

Although lipooligosaccharides (LOSs) are recognized as major parietal component in many mycobacterial species, their involvement in the host-pathogen interactions have been scarcely documented. In particular, the biological implications arising from the high degree of structural species-specificity of these glycolipids remain largely unknown. Growing recognition of the Mycobacterium marinum-Danio rerio as a specific host-pathogen model devoted to the study of the physiopathology of mycobacterial infections prompted us to elucidate the structure to function relationships of the elusive end-product, LOS-IV, of the LOS biosynthetic pathway in M. marinum. Combination of physico-chemical and molecular modelling methods established that LOS-IV resulted from the differential transfer on the caryophillose-containing LOS-III of a family of a very unusual N-acylated monosaccharides, naturally present as different diastereoisomers. In agreement with the partial loss of pathogenecity previously reported in a LOS-IV-deficient M. marinum mutant, we demonstrate here that the presence of this terminal monosaccharide was required to confer to LOS-IV important biological functions that include macrophage activating properties.