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Accueil du site > Production scientifique > Differentiating fragmentation pathways of cholesterol by Two-Dimensional Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

Differentiating fragmentation pathways of cholesterol by Two-Dimensional Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

Date de publication: 17 juillet 2015

M. A. van Agthoven, M. P. Barrow, L. Chiron, M.-A. Coutouly, D. Kilgour, C. A. Wootton, Juan Wei, A. Soulby, M.-A. Delsuc, C. Rolando, P. B. O’Connor
J. Am. Soc. Mass Spectrom. 26 2105-2114 (2015). DOI

Travail réalisé sur le site de l’Université de Lille 1, Sciences et Technologies.

Abstract

Two-dimensional Fourier transform ion cyclotron resonance mass spectrometry is a data-independent analytical method which records the fragmentation patterns of all the compounds in a sample. This study shows the implementation of atmospheric pressure photoionization with two-dimensional Fourier transform ion cyclotron resonance mass spectrometry. In the resulting two-dimensional mass spectrum, the fragmentation patterns of the radical and protonated species from cholesterol are differentiated. This study shows the use of fragment ion lines, precursor ion lines and neutral loss lines in the 2D mass spectrum to determine fragmentation mechanisms of known compounds and to gain information on unknown ion species in the spectrum. In concert with high resolution mass spectrometry, two-dimensional Fourier transform ion cyclotron resonance mass spectrometry can be a useful tool for the structural analysis of small molecules.