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Accueil du site > Production scientifique > Structure of sodiated polyglycines

Structure of sodiated polyglycines

Date de publication: 10 avril 2012

O.P. Balaj, D. Semrouni, V. Steinmetz, E. Nicol, C. Clavaguéra, G. Ohanessian
Chemistry 18(15) 4583-92 (2012). DOI

Travail réalisé sur le site de l’Université Paris-Sud.


The intrinsic folding of peptides about a sodium ion has been investigated in detail by using infrared multiple photon dissociation (IRMPD) spectroscopy and a combination of theoretical methods. IRMPD spectroscopy was carried out on sodiated polyglycines G(n)-Na(+) (n=2-8), in both the fingerprint and N-H/O-H stretching regions. Interplay between experimental and computational approaches (classical and quantum) enables us to decipher most structural details. The most stable structures of the small peptides up to G(6)-Na(+) maximize metal-peptide interactions with all peptidic C=O groups bound to sodium. In addition, direct interactions between peptide termini are possible for G(6)-Na(+) and larger polyglycines. The increased flexibility of larger peptides leads to more complex folding and internal peptide structuration through γ or β turns. A structural transition is found to occur between G(6)-Na(+) and G(7)-Na(+), leading to a structure with sodium coordination that becomes tri-dimensional for the latter. This transition was confirmed by H/D exchange experiments on G(n)-Na(+) (n=3-8). The most favorable hydrogen-bonding pattern in G(8)-Na(+) involves direct interactions between the peptide termini and opens the way to salt-bridge formation ; however, there is only good agreement between experimental and computational data over the entire spectral range for the charge solvation isomer.