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Accueil du site > Production scientifique > Proteomic study of human bronchial epithelial cells exposed to SiC nanoparticles

Proteomic study of human bronchial epithelial cells exposed to SiC nanoparticles

Date de publication: 26 octobre 2011

C. Tokarski, S. Hirano, C. Rolando
J. Phys. Conf. Ser. 304 012088/1-012088/5 (2011). DOI

Travail réalisé sur le site de l’Université de Lille 1, Sciences et Technologies.

Abstract

The presented work proposes an optimized methodol. for the study of cell exposure to nanomaterials at protein level. The study was investigated on proteins extd. from human bronchial epithelial cells exposed and non-exposed to silicon carbide nanoparticles (SiC). The anal. strategy was based on high resoln. measurement using Fourier transform mass spectrometer 9.4 T. The methodol. proposed succeeds in identifying over 300 proteins ; most of the identified proteins are present in both exposed and non exposed cells to SiC nanoparticles. More interestingly, cytokines as Macrophage migration inhibitory factor protein could be identified only in the cells exposed to SiC nanoparticles indicating cell inflammatory response.